Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
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چکیده
منابع مشابه
Testing of an Antitumor Enzyme L-lysine-A-oxidase from Trichoderma Harzianum Rifai F-180
An enzyme L-lysine-A-oxidase has been obtained from Trichoderma harzianum Rifai F-180. The analysis of serum from CBA mice immunized five times with native L-lysine-A-oxidase intravenously, at a dose of 35 U/kg, showed that the preparation had virtually no effect on the immune response to Tdependent antigen. L-lysine-A-oxidase at a dose of 35 U/kg administered twice intradermally has no effect ...
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Effects of induced mutation via gamma irradiated with 250 Gy dose (in Nuclear Agriculture Research School- Nuclear Science and Technology Research Institute) on Trichoderma harzianum conidiaon chitinase activity by STS molecular marker have been evaluated in this study. Among 20 irradiated mutants which selected via improved antagonistic capability against Rhizoctonia sola...
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A minimal medium which allowed the sustained, rapid growth of Zymomonas mobilis and the isolation of a range of auxotrophic mutants was developed.
متن کاملInvestigation of Mutation in Chitinase Gene in Gamma Radiated Mutants of Trichoderma Harzianum by STS Marker
Effects of induced mutation via gamma irradiated with 250 Gy dose (in Nuclear Agriculture Research School- Nuclear Science and Technology Research Institute) on Trichoderma harzianum conidiaon chitinase activity by STS molecular marker have been evaluated in this study. Among 20 irradiated mutants which selected via improved antagonistic capability against Rhizoctonia sola...
متن کاملIsolation and characterization of mutants of Saccharomyces cerevisiae auxotrophic and conditionally auxotrophic for 5'-dTMP.
An improved method for isolation of yeast m utants auxotrophic for 5'-dTM P is presented. The procedure employs the two folic acid antagonists aminopterin and sulfanilamide (SA A). Selec tiveness of the procedure depends on concentration of SAA and time of incubation. 44 mutants auxotrophic and 3 conditionally auxotrophic for 5'-dTMP were isolated. All belong to one complementation group. The ...
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ژورنال
عنوان ژورنال: Revista de Microbiologia
سال: 1999
ISSN: 0001-3714
DOI: 10.1590/s0001-37141999000100008